Research Article
Baicalin Ameliorates DSS-Induced Colitis by Protecting Goblet Cells through Activating NLRP6 Inflammasomes
| | Gene | Primer | Sequence (5′-3′) |
| | β-Actin | F | CTCATGAAGATCCTGACCGAG | | R | AGTCTAGAGCAACATAGCACAG |
| | IL-6 | F | CCACTTCACAAGTCGGAGGCTTA | | R | AGTGCATCATCGTTGTTCATAC |
| | TNF-α | F | AAGGCCGGGGTGTCCTGGAG | | R | AGGCCAGGTGGGGACAGCTC |
| | IL-1β | F | GAAATGCCACCTTTTGACAGTG | | R | TGGATGCTCTCATCAGGACAG |
| | MUC2 | F | TGTGTTTCAGGCTCCATCAC | | R | TGCAGCCATTGTAGGAAATC |
| | NLRP6 | F | TGGAGCCTCCTGCTCCAG | | R | GCGAGGCCACATCTCGAATA |
| | Caspase-1 | F | CCTGTCAGGGGCTCACTTTT | | R | GGTCACCCTATCAGCAGTGG |
| | ASC | F | TGACAGTGCAACTGCGAGAA | | R | GTGAGCTCCAAGCCATACGA |
| | IL-18 | F | GGCTGCCATGTCAGAAGACT | | R | GTCTGGTCTGGGGTTCACTG |
| | IL-10 | F | CTTACTGACTGGCATGAGGATCA | | R | GCAGCTCTAGAGCATGTGG |
| | Zo-1 | F | GTCCCTGTGAGTCCTTCAGC | | R | TAGGGTCACAGTGTGGCAAG |
| | E-cadherin | F | AACCCAAGCACGTATCAGGG | | R | ACTGCTGGTCAGGATCGTTG |
| | Claudin-5 | F | CCTTCCTGGACCACAACATC | | R | CGCCAGCACAGATTCATACA |
| | Claudin-4 | F | ACGTCATCCGCGACTTCTAC | | R | TTGTCGTTGCTACGAGGTGG |
| | Claudin-2 | F | CAAAGCCAAGAGTGAGTTCAA | | R | TCATTCTACCCTCAGCAGCA |
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