Curcumin Alleviates D-Galactose-Induced Cardiomyocyte Senescence by Promoting Autophagy via the SIRT1/AMPK/mTOR Pathway

Figure 1

Curcumin inhibited D-galactose-induced senescence and decreased ROS in cardiomyocytes. (a) Primary neonatal rat cardiomyocytes were treated with D-gal (10 g/l). The suppression effect of curcumin on primary neonatal rat cardiomyocyte senescence shown by SA-β-galactosidase staining (n = 5). Scale bar: 100 μm. (b) SA-β-galactosidase-positive cells. (c) Curcumin inhibited the expression of the senescence markers p53 and p16 in senescent cardiomyocytes, as observed using western blot (n = 5). (d) p53 expression. (e) p16 expression. (f) Curcumin reduces intracellular ROS levels in cardiomyocytes as observed using the DCFH-DA probe staining, n = 5, scale bar: 100 μm. (g) Statistical results for ROS production. vs. control. , , vs. D-gal. The data are expressed as mean ± SEM.

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