Evidence-Based Complementary and Alternative Medicine

Research Article

Changes in the Chemical Components of Processed Rehmanniae Radix Distillate during Different Steaming Times

Table 1

Quantitative analysis results of chemical components in Dis1–Dis9 by LC-MS.


Compound no.	Identification	t_R (min)	Formula	m/z	ppm	MS2	M+	Steaming times	Source

1	Dihydrocatalpol	2.47	C₁₅H₂₄O₁₀	382.1691	−4.376	185.0804 [M-C₆H₁₂O₆-H₂O]⁺ 167.0699 [M-C₆H₁₂O₆-2H₂O]+	[M + NH₄]⁺	Dis1-Dis2	Catalpol
2	Compound 2	2.56	C₉H₈O₃	182.0803	−4.996	96.0439, 87.0436	[M + NH₄]⁺	Dis1–Dis9	Catalpol
3	Iridoids	3.53	C₉H₁₃O₅	201.0748	−4.874	183.0643 [M-H₂O]⁺ 165.0538 [M-2H2O]+	[M + H]⁺	Dis1–Dis9	Catalpol
4	1,5-Dialdehyde
5	Catalpol	3.91	C₁₅H₂₂O₁₀	380.1536	−4.268	183.0643 [M-C₆H₁₂O₆]⁺ 165.0538 [M-C₆H₁₂O₆-H₂O]⁺	[M + NH₄]⁺	Dis1-Dis2	Prototype components
6	5-Hydroxymethylfurfural	6.73	C₆H₇O₃	127.0383	−4.358	109.0279 [M-H₂O]⁺ 81.0331 [M-H₂O-CO]⁺	[M + H]⁺	Dis1–Dis9	Saccharides
7	Monomelittoside	7.36	C₁₅H₂₂O₁₀	380.1536	−3.926	327.1071 [M-2H₂O]⁺ 165.0542 [M-C₆H₁₂O₆-H₂O]⁺	[M + NH₄]⁺	Dis1–Dis4	Prototype components
8	Rehmannioside D	8.40	C₂₇H₄₂O₂₀	704.2578	−4.202	489.1582 [M-C₆H₁₂O₆]⁺ 471.1472 [M-C₆H₁₂O₆-H₂O]⁺	[M + NH₄]⁺	Dis1–Dis9	Prototype components
9	Rehmannioside A	8.70	C₂₁H₃₂O₁₅	542.2057	−4.160	345.1170 [M-C₆H₁₂O₆]⁺ 183.0648 [M-2C₆H₁₂O₆]⁺	[M + NH₄]⁺	Dis1–Dis9	Prototype components
10	Leonuride	9.76	C₁₅H₂₄O₉	366.1745	−3.790	169.0851 [M-C₆H₁₂O₆]⁺ 151.0746 [M-C₆H₁₂O₆-H₂O]⁺	—	Dis1	Prototype components
11	Leonuride aglycone	11.35	C₉H₁₃O₃	169.0853	−5.682	151.0746 [M-H₂O]⁺ 141.0539 [M-C₂H₄]⁺	[M + H]⁺	Dis1–Dis9	Leonuride
12	Verbasoside	11.77	C₂₀H₃₀O₁₂	480.2054	−4.585	317.1219 [M-C₆H₁₀O₄]⁺ 309.1169 [M-C₈H₉O₃]⁺	[M + NH₄]⁺	Dis1–Dis9	Acteoside
13	Cistanoside F	13.06	C₂₁H₂₈O₁₃	506.1847	−4.141	—	—	Dis1–Dis3	Acteoside
14	Compound 14	13.69	C₉H₁₅O₅N	217.0959	3.720	171.0909 [M-CH₂O₂]⁺ 144.0802 [M-CH₂O₂-C₂H₂]⁺	[M + NH₄]⁺	Dis1–Dis9	Catalpol
15	Compound 15	14.89	C₁₂H₁₃O₅	237.0748	−4.218	191.0694 [M-CH₂O₂]⁺ 163.0382 [M-C₃H₆O₂]+	[M + H]⁺	Dis2–Dis9	Geniposide
16	Hydroxytyrosol	15.85	C₈H₁₁O₃	155.0695	−4.228	—	—	Dis1	Acteoside
17	Echinacoside	20.57	C₃₅H₄₆O₂₀	804.2881	−4.873	625.2086 [M-C₆H₁₀O₅]⁺ 471.1472 [M-C₆H₁₀O₅-C₈H₉O₃]⁺	[M + NH₄]⁺	Dis1–Dis9	Prototype components
18	Rehmaionoside A	22.53	C₁₉H₃₄O₈	408.2574	−4.271	211.1684 [M-C₆H₁₂O₆]⁺ 193.1579 [M-C₆H₁₂O₆-H₂O]⁺	[M + H]⁺	Dis1–Dis6	Prototype components
19	Cistanoside A	22.79	C₃₆H₄₈O₂₀	818.3037	−4.899	639.2257 [M-C₆H₁₀O₅]⁺	—	Dis1–is9	Prototype components
20	Acteoside	25.26	C₂₉H₃₆O₁₅	642.2363	−4.633	479.1527 [M-C₆H₁₀O₃]⁺ 325.0905 [M-C₁₄H₂₀O₆]⁺	[M + NH₄]⁺	Dis1–Dis9	Prototype components
21	Isoacteoside	26.70	C₂₉H₃₆O₁₅	642.2380	−1.877	479.1527 [M-C₆H₁₀O₃]⁺ 325.0905 [M-C₁₄H₂₀O₆]⁺	[M + NH₄]⁺	Dis1–Dis9	Acteoside