Development and Optimization of Nanoemulsion from Ethanolic Extract of Centella asiatica (NanoSECA) Using D-Optimal Mixture Design to Improve Blood-Brain Barrier Permeability

Table 2

Experimental data of actual and predicted values of particle size of VCO-based nanoemulsion.

Standard order	Run order	Independent variables		Particle size (nm)
		Deionized water (%)	VCO (%)	Actual^1	Predicted
1	4	10.00	10.00	259.93 ± 0.577	274.61
2	1	80.00	10.00	129.76 ± 1.762	146.49
3	5	10.00	80.00	679.30 ± 1.315	640.48
4	12	80.00	80.00	500.23 ± 0.252	567.87
5	11	45.00	80.00	400.45 ± 2.261	507.06
6	10	80.00	45.00	160.20 ± 6.102	169.31
7	7	45.00	27.50	150.36 ± 10.344	70.950
8	14	10.00	45.00	253.20 ± 3.710	269.68
9	3	27.50	62.50	250.80 ± 2.301	313.66
10	15	62.50	62.50	326.60 ± 5.803	270.41
11	6	45.00	10.00	145.65 ± 2.301	113.44
12	16	10.00	10.00	259.93 ± 5.803	274.61
13	9	80.00	10.00	129.76 ± 0.987	146.49
14	2	10.00	80.00	678.10 ± 2.984	640.48
15	13	80.00	80.00	500.23 ± 3.176	567.87
16	8	80.00	45.00	140.35 ± 2.506	169.31

¹Data obtained are means of triplicates.