Development and Optimization of Nanoemulsion from Ethanolic Extract of Centella asiatica (NanoSECA) Using D-Optimal Mixture Design to Improve Blood-Brain Barrier Permeability

Table 6

Optimization data on percentage (%) of SECA-K018 in VBN: F2.

No	Formulations (% of SECA-K018)	Particle size (nm)	Zeta potential (ZP)	Polydispersity index (PDI)	AChE-inhibitory activity (IC50) µg/mL
1	VBN: F2: 10,000: (0.5%)	147.50 ± 3.236	−19.567 ± 0.015	0.386 ± 5.123	>1000
2	VBN: F2: 10,000: (1%)	92.397 ± 4.080	−27.833 ± 0.874	0.259 ± 0.070	>1000
3	VBN: F2: 10,000: (2%)	127.833 ± 8.280	−24.9 ± 0.011	0.493 ± 4.681	974.9 ± 5.33
4	VBN: F2: 10,000: (5%)	192.767 ± 13.249	−1.375 ± 1.005	0.809 ± 0.057	>1000