Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells

Figure 2

ALR and PALR inhibited the proliferation of HMCs. (a) The morphology of ALR (10 mg/ml), PALR (0.1 mg/ml), and LPS-treated (100 μg/ml) HMCs for 24 h. The control group was added with 100 μl of DMEM, while other groups were each added with 100 μl of 100  μg/ml LPS. After 12 h of stimulation, the three drug groups were added with 100 μl of ALR, PALR, and MP, respectively, however, the control group and the LPS group were both added to 100 μl of DMEM, followed by 24 h of intervention. Images were taken using a microscope (200 × magnification). (b) The number of the HMCs counted using a Coulter Counter. All values are expressed in mean ± SD (n = 3; ^## versus control group; versus LPS group). (c) The inhibition rate of HMCs in each group. All values are expressed in mean ± SD (n = 6; ^## versus control group; versus LPS group).

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