Aqueous Extract and Polysaccharide of Aconiti Lateralis Radix Induce Apoptosis and G0/G1 Phase Cell Cycle Arrest by PI3K/AKT/mTOR Signaling Pathway in Mesangial Cells

Figure 4

The induction of cell apoptosis arrest by ALR and PALR in HMCs. (a) Representative images of cell apoptosis. Flow cytometry analysis revealed that ALR (10 mg/ml) and PALR (0.1 mg/ml) induced the apoptosis of HMCs, as determined by AnnexinV-PE/7AAD. (b) The apoptotic rate of HMCs in each group. All data are depicted as mean ± SD (n = 3; ^## versus control group, versus LPS group). (c–j) The protein expressions of Bax, cleaved caspase-8/caspase-8, and cleaved caspase-3/caspase-3 were analyzed using Western blot. HMCs in (c–f) were induced by LPS. However, there was non-LPS induction in (g–j). All data are depicted as mean ± SD (n = 3; ^# or ^## versus control group, or versus LPS group).

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