Panax Notoginseng Saponins Regulate Transforming Growth Factor-β1 through MAPK and Snail/TWIST1 Signaling Pathway to Inhibit Epithelial-Mesenchymal Transition of Pulmonary Fibrosis in A549 Cells

Figure 5

PNS inhibits epithelial-mesenchymal transition through the MAKP signaling pathway. To study the expression level of the MAPK signal pathway under different time conditions stimulated by TGF-β1 (a). . . vs no TGF-β1 added. (b) Next, 72 hours was selected as the stimulation time of TGF-β1, and Western blotting was used to detect the protein expression of p38, p-p38, Erk, p-Erk, JNK, and p-JNK related signaling molecules in the MAPK signaling pathway. . vs. Control group. ^＃. ^＃＃. ^＃＃＃ vs. TGF-β1 group. (c) Then select immunofluorescence technology to further detect the fluorescence of p-p38, p-Erk, and p-JNK signaling molecules related to the MAPK signaling pathway (magnification, ×500). (d) After adding 3 kinds of MAPK signal pathway inhibitors (SB203580/PD98059/SP600125), Western blotting was used to detect E-cad, FN, and Vim protein related to EMT. . . vs. Control group. ^＃. ^＃＃. ^＃＃＃ vs. TGF-β1 group.

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