Yunnan Baiyao Ameliorates Rheumatoid Arthritis in Rats by Shifting the Th17/Treg Cell Balance and Preventing Osteoclast Differentiation

Figure 4

Effects of YNB on RANKL‐induced osteoclastogenesis. (a, b) RAW264.7 cells induced by RANKL (100 ng/mL) were treated with YNB (5, 10, or 20 μg/mL) for 7 days, fixed, and stained with a TRAP staining kit to detect TRAP⁺ cells (magnification ×200). (c) RT‐PCR analysis of TRAF6, NF-κB, and NFATC1 mRNA. (d) Protein levels of phospho-NF-κB, NF-κB, TRAF6, and NFATC1 were evaluated by western blotting. Data are presented as mean ± SD. ^a vs. control; ^b vs. RANKL; ^c vs. RANKL +5 μg/mL; and ^d vs. RANKL +10 μg/mL.

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