Live-Imaging Analysis of Target Vessels and Nitric Oxide Production Associated with Gosha-Jinki-Gan and Keishi-Bukuryo-Gan: Two Herbal Preparations with Clinically Proven Blood Flow-Improving Effects but with Different Traditional Clinical Indicative Patterns

Figure 5

Typical live-imaging images of rat mesenterial regions. Representative images of a typically analyzed rat mesenterial region (a) Areas U6, M6, and L6 correspond to the upstream, midstream, and downstream of a mesenteric artery perfusion area, respectively. Panels (b) and (c) depict a series of representative images before and at 120 min after GJG administration in the mesenteric arterioles and capillaries. Before GJG administration Panel b, cell-free layers were extensively observed in the capillaries (arrow and arrowhead), whereas after GJG administration Panel c, erythrocytes were continuously observed in the vessels and cell-free layers were absent. The imaging video file used for the analysis is provided in Additional Material 5.

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