Untargeted Metabolomics Analysis of Crocus cancellatus subsp. damascenus (Herb.) B. Mathew Stigmas and Their Anticarcinogenic Effect on Breast Cancer Cells

Table 3

IC₅₀ values of the stigmas ethanol extract against MDA-MB-231 and MCF-7 in monolayer (2D) and spheroid (3D) cultures.

Type of culture	IC50 (μg/mL)
	MDA-MB-231		MCF-7
	24 hours	72 hours	24 hours	72 hours
Two-dimensional (2D) monolayers	97.54 ± 9.66^a,b	69.06 ± 6.55^a,b	131.44 ± 8.36^a,b	78.70 ± 9.09^a,b
Three-dimensional (3D) spheroid	325.13 ± 15.21^a,b	155.45 ± 14.22^a,b	>400	>400

The concentrations of the extract that could provide 50% cytotoxic activity (IC₅₀) were calculated from the graph that plotted the inhibition percentage against different concentrations. ^aIC₅₀ values are significantly different between 24 and 72 Hours. ^bThe IC₅₀ values are significantly different between 2D and 3D cultures.