FAM117A Is a New Prognostic Marker of Lung Adenocarcinoma and Predicts Sensitivity to PD0332991

Figure 3

Effects of FAM117A expression in lung cancer cells. (a) Images of western blot results of FAM117A expression in lung cancer cell lines (NCI-H1975 and A549) upon transfection with short hairpin RNA oligos targeting the FAM117A transcript, β-actin was used as an internal control. (b) Quantitative analysis of the grey scale of FAM117A expression by western blot. (c) Quantitative real-time RT-PCR of FAM117A expression in lung cancer cells transfected with shRNA oligo targeting its transcript. (d, e) Images of colony formation for lung cancer cells knockdown with FAM117A. (f, g) Quantitative results of the colony formation assay in lung cancer cells. (h, i) Lung cancer cell proliferation rate as confirmed by CCK-8 based assay for 3 days post seeding in 96-well plate. (j) Images of western blot results of FAM117A overexpression in lung cancer cell lines (NCI-H1975 and A549) upon transfection with Flag-FAM117A, β-actin was used as an internal control. (k, l) Lung cancer cell proliferation rate as confirmed by CCK-8 based assay for 3 days postseeding in 96-well plate. (m, o) Images of colony formation and quantitative results for lung cancer cells overexpressed with the empty vector of Flag-FAM117A.

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