FAM117A Is a New Prognostic Marker of Lung Adenocarcinoma and Predicts Sensitivity to PD0332991

Figure 4

FAM117A regulates the cell cycle progress of lung cancer cells. (a–c) Representative images of the cell cycle profile determined by flow cell cytometry in lung cancer cells transfected with negative control oligo or shRNA oligos targeting the FAM117A transcript. Quantitative analysis results were shown in B (NCI-H1975) and C (A549). (d–f) Representative images of the cell cycle profile determined by flow cell cytometry in lung cancer cells transfected with an empty vector or Flag-FAM117A transcript. Quantitative analysis results were shown in B (NCI-H1975) and C (A549). (g–h) Representative images of EDU labelling lung cancer cells captured by an immunofluorescence microscope. (h, i) Quantitative analysis results of western blot results of FAM117A expression in lung cancer cell lines (NCI-H1975 and A549) upon transfection with Flag-Vector or Flag-FAM117A. Cell cycle-related markers were detected as indicated, and β-actin was used as an internal control. (j, k) Quantitative analysis result of the grey scale of the western blot result.

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