Research Article
The Evaluation of Xiaozeng Qianggu Tablets for Treating Postmenopausal Osteoporosis via up-Regulated Autophagy
Table 1
Forward and reverse primers used in reverse transcription quantitative PCR.
| | Gene ID | Target gene | Primer sequence (5′-3′) | Tm (°C) | Product length (Bp) |
| | NM_031144.3 | β-actin:F | ACAACCTTCTTGCAGCTCCTC | 60 | 200 | | β-actin:R | CTGACCCATACCCACCATCAC |
| | NM_001108341.1 | ULK1:F | ATGGTGGAGACCTGGCTGAC | 52.5 | 203 | | ULK1:R | AATCTTGACTCGGATGTTGCTG |
| | XM_017596950.1 | Beclin-1:F | TGAGGAGCAGTGGACAAAGG | 60 | 75 | | Beclin-1:R | TGTGAGGACACCCAAGCAAG |
| | NM_022867.2 | LC3B:F | AGAGCGATACAAGGGTGAGAAG | 57.1 | 134 | | LC3B:R | AGAAGGCTTGGTTAGCATTGAG |
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The all primers were rat species. F, Forward primer, R, Reverse primer. Tm, Temperature of melting.
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