Effect of PhenylEthanol Glycosides from Cistanche Tubulosa on Autophagy and Apoptosis in H22 Tumor-Bearing Mice

Figure 4

Induction of autophagy in H22 tumor-bearing mice by CPhGs. (a) p62 and LC3B-II expression of tumor from mice were assayed by IHC (100×, scale bar is 100 μm). (b) The protein levels of p62 and LC3B-II in of tumor issue were calculated depending on IHC. (c) Ultrastructure of autophagic vesicles in tumor tissue of H22 tumor-bearing mice by transmission electron microscopy (3000x/10000x, scale bar is 500 nm and 2 μm, respectively). (d) Autophagy-associated protein p62 and LC3B-II detected by western blot. (e, f) p62 and LC3B-II protein levels in tumor tissues were calculated using western blot. The results are shown as the mean ± SD vs. model group, , , vs. normal group, ^#.

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