Effect of PhenylEthanol Glycosides from Cistanche Tubulosa on Autophagy and Apoptosis in H22 Tumor-Bearing Mice

Figure 5

Effect of autophagy regulation on apoptosis induced by CPhGs in H22 tumor-bearing mice. (a) Ultrastructure of autophagic vesicles in tumor tissue of H22 tumor-bearing mice by transmission electron microscopy (3000x/10000x, scale bar is 500 nm and 2 μm, respectively). (b) p62 and LC3B-II expression of tumor from mice were assayed by IHC (100x, scale bar is 100 μm). (c) Comparison of the size of unisolated tumors in each group of mice. (d, e) Protein levels of p62 and LC3B-II in of tumor issue were calculated depending on IHC. (f) Comparison of the size of isolated tumors in each group. (g, i) Trend graph of tumor growth in each group during the experiment and the comparison of the volume size of isolated tumors in each group. (j) Cleaved caspase-3 and cleaved-PARP expression of tumor from mice were assayed by IHC (100x, scale bar is 100 μm) and TUNEL staining of tumor tissues was used to observe the occurrence of apoptosis in tumor cells H22 tumor-bearing mice (scale bar is 100 μm). (h) Apoptosis rate of H22 tumor-bearing mice tumor tissue was calculated according to TUNEL staining. (k) The protein levels of cleaved caspase-3 and cleaved-PARP in of tumor issue were calculated depending on IHC. The results were shown as the mean ± SD vs. model group, ,, vs. CPhGs group, ^#, ^## vs. HCQ group, ^a, ^aa vs. Rapamycin group, ^b, ^bb.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)