Buyang Huanwu Decoction: A Traditional Chinese Medicine, Promotes Lactate-Induced Angiogenesis in Experimental Intracerebral Hemorrhage

Figure 1

LC-MS analysis of three main components (amygdalin, hydroxysafflor yellow A and calycosin) of (a) standard agents, (b) decoction of BYHWD, (c) plasma sample of the ICH + BYHWD group and (d) brain sample of the ICH + BYHWD group. (e) The diagram of the experimental timeline. (f and g) The rats in the OXA group showed significantly higher mNSS (3 d, 7 d, and 14 d), and lower lactate levels (7 d and 14 d) than those in the ICH group. The neurological function of the BYHWD-treated rats markedly outperformed that of rats subjected to ICH on days 3, 7, and 14. BYHWD induced lactate accumulation in ICH. The rats in the BYHWD group showed better performances in the behavior test (3 d, 7 d and 14 d) and higher lactate levels than their inhibitor-treated counterparts, F: n = 6/group, G: n = 5/group. (h) Representative figures for PCNA⁺ nuclei located in vWF⁺ microvessels. (i) OXA significantly reduced the number of PCNA⁺/vWF⁺ cells at each time point after ICH. BYHWD significantly enhanced angiogenesis after ICH. Compared with those in the BYHWD-treated group, significantly decreased numbers of PCNA⁺ nuclei in vWF⁺ vessels were observed in the BYHWD + OXA-treated group, n = 5/group. and . Scale bar = 100 μm.

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