Therapeutic Benefits of Pomegranate Flower Extract: A Novel Effect That Reduces Oxidative Stress and Significantly Improves Diastolic Relaxation in Hyperglycemic In Vitro in Rats

Figure 6

(a) The effect of high glucose and PFP on ACh-mediated relaxation. (b) The effect of different concentrations of PFP incubated with high glucose on ACh-mediated endothelium-dependent relaxation. (c) The effect of atropine and L-NAME on ACh-mediated relaxation of thoracic aortic rings incubated by PFP and high glucose (x̅ ± SD, n = 6). and vs. control. Note. HG: high glucose; HG + PFP: vascular rings were incubated for 6 hours with K-H solution containing 10 mg/L PFP and 44 mM D-glucose; HG + PFP + atropine: vascular rings were pretreated with 0.1 mM atropine for 30 minutes and then incubated with K-H solution containing 10 mg/L PFP and 44 mM D-glucose for 6 hours; HG + PFP + L-NAME: vascular rings were pretreated with 0.1 mM L-NAME for 30 minutes and consequently incubated with K-H solution containing 10 mg/L PFP and 44 mM D-glucose for 6 hours. The diastolic responses mediated by ACh and SNP of thoracic aortic rings in each group were measured.

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