Isoflavones from Semen Sojae Preparatum Improve Atherosclerosis and Oxidative Stress by Modulating Nrf2 Signaling Pathway through Estrogen-Like Effects

Table 2

Primer sequences.

Gene	Endothelial cell	Gene	Mice
Nrf2	(F) 5′-TCCAGTCAGAAACCAGTGGAT-3′	Nrf2	(F) 5′-TCCAGTCAGAAACCAGTGGAT-3′
	(R) 5′-GAATGTCTGCGCCAAAAGCTG-3′		(R) 5′-GAATGTCTGCGCCAAAAGCTG-3′
HO-1	(F) 5′-AAGACTGCGTTCCTGCTCAACT-3′	HO-1	(F) 5′-AAGACTGCGTTCCTGCTCAACT-3′
	(R) 5′-AAAGCCCTACAGCAACTGTCG-3′		(R) 5′-AAAGCCCTACAGCAACTGTCG-3′
NQO1	(F) 5′-GAGAAGAGCCCTGATTGT-3′	NQO1	(F) 5′-GAGAAGAGCCCTGATTGT-3′
	(R) 5′-AAAGGACCGTTGTCGTAC-3′		(R) 5′-AAAGGACCGTTGTCGTAC-3′
GAPDH	(F) 5′-AGAAGGCTGGGGCTCATTTG-3′	β-actin	(F) 5′-AGAAGGCTGGGGCTCATTTG-3′
	(R) 5′-AGGGGCCATCCACAGTCTTC-3′		(R) 5′-AGGGGCCATCCACAGTCTTC-3′