Hyunganol II Exerts Antiadipogenic Properties via MAPK-Mediated Suppression of PPARγ Expression in Human Bone Marrow-Derived Mesenchymal Stromal Cells

Figure 3

Effect of HNG on the protein expression of adipogenic marker genes, PPARγ, C/EBPα, and SREBP1c in differentiating hBM-MSCs. (a) The whole cell and (b) nuclear protein levels were analyzed with Western blotting. β-actin and lamin B1 were used as internal controls for whole cell and nuclear protein levels, respectively. (c) Intracellular PPARγ protein was stained with immunofluorescence staining (green) and DAPI nuclear staining (blue) was used as viable cell control. ^a–eBars with identical letters indicate no statistical significance (), while different letters indicate otherwise.

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