Vascular Protective Effects of Malus toringoides (Rehd.) Hughes Extracts and their Mechanism in Diabetic Rats and HUVECs

Figure 6

Effects of EMT on NF-κB activation in rat aortic histological sections and HUVECs. (a) Immunohistochemical detection of NF-κB activation in aortic sections from a diabetic rat model established for 6 weeks and administered treatment for 8 weeks. (b) Western blot analyses of IκB-α and phospho-IκB-α expression in the cytoplasm and NF-κB p65 expression in the nucleus were performed as described in the materials and methods section, and representative blots of 3 independent experiments are shown. (c) NF-κB p65 nuclear translocation was measured by immunofluorescence. The data represent the mean values of 3 experiments ± SD. ^# or ^## compared to the group treated with high glucose.

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