Inhibitory Effects of Rhein on Renal Interstitial Fibrosis via the SHH-Gli1 Signal Pathway

Figure 4

Rhein inhibits SHH signaling pathway in NRK-49F cells activated by SHH. Western blot and qPCR analysis show the expression of Gli1 and Snail increased in NRK-49F cells induced by SHH (100 ng·ml⁻¹) for 48 hours. The NRK-49F cells were incubated with SHH in the presence of rhein (1 ng·ml⁻¹) or CNP (1 ng·ml⁻¹), and the expression of Gli1 and Snail mRNA and protein decreased. Data are presented as mean ± SEM. , .