Zhenzhu Xiaoji Decoction Induces Autophagy and Apoptosis Cell Death in Liver Cancer Cells through AKT/mTOR and JAK2/STAT3 Signaling Pathway

Table 2

Primer sequences of qRT-PCR.

Genes	Sequences
GAPDH	F: 5′’-GGAGCGAGATCCCTCCAAAAT-3′’
	R:5′’-GGCTGTTGTCATACTTCTCATGG-3′’
JAK2	F: 5′’-GTGTGGAGATGTGCCGGTATGAC-3′’
	R: 5′’-GAT TACGCCGACCAGCACTGTAG-3′’
STAT3	F: 5′’-CGGAGAAGCATCGTGAG TGAGC-3′’
	R: 5′’GTTGCCTCTTCCAGTCA G-3′’
AKT	F: 5′’-GTCATCGAACGCACCTTCCAT-3′’
	R: 5′’-AGCTTCAGGTACTCAAACTCGT-3′’
mTOR	F: 5′’-ACCGGCACACATTTGAAGAAG-3′’
	R: 5′’-CTCGTTGAGGATCAGCAAGG-3′’
LC3	F: 5′’-AACATGAGCGAGTTGGTCAAG-3′’
	R: 5′’-GCTCGTAGATGTCCGCGAT-3′’
P62	F: 5′’-GACTACGACTTGTGTAGCGTC-3′’
	R: 5′’-AGTGTCCGTGTTTCACCTTCC-3′’
BECLIN1	F: 5′’-GGTGTCTCTCGCAGATTCATC-3′’
	R: 5′’-TCAGTCTTCGGCTGAGGTTCT-3′’
BAX	F: 5′’-CCCGAGAGGTCTTTTTCCGAG-3′’
	R: 5′’-CCAGCCCATGATGGTTCTGAT-3′’
BCL-2	F: 5′’-GAACTGGGGGAGGATTGTGG-3′’
	R: 5′’-GCATGCTGGGGCCATATAGT-3′’