The Antitumor Effect of TPD52L2 Silencing on Oxaliplatin-Resistant Gastric Carcinoma Is Related to Endoplasmic Reticulum Stress In Vitro

Figure 2

Validation of small interfering (si) RNA-mediated silencing of TPD52L2. The transfection of SGC-7901-OXA and MGC-803-OXA cells with negative-control siRNA (siCon) or three TPD52L2 siRNAs (siRNA-1, siRNA-2, and siRNA-3) for two days. (a) The mRNA expression of TPD52L2 was determined via qRT-PCR, normalized to GAPDH expression, and depicted as fold-change relative to the control siRNA group. (b) The protein expression level of TPD52L2 was determined through immunoblotting with GAPDH as the loading control. (c) Quantification of TPD52L2 protein level normalized to GAPDH. All values are indicated as mean ± SD of three replicates. value <0.01 and value <0.001 compared to the siCon group.

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