Comparative Pharmacokinetics of Seven Major Compounds in Normal and Atherosclerosis Mice after Oral Administration of Simiao Yong’an Decoction

Table 5

Stability of seven target analytes in QC plasma samples (mean ± SD, n = 5).

Compounds	QC (ng/ml)	Postpreparative stability 24 h in the autosampler (4°C)		Short-term stability 24 h in room temperature (20°C)		Freeze-thaw stability 3 freeze-thaw cycles		Long-term stability −20°C for 30 d
		Calc. conc. (ng/ml)	Accuracy RE (%)	Calc. conc. (ng/ml)	Accuracy RE (%)	Calc. conc. (ng/ml)	Accuracy RE (%)	Calc. conc. (ng/ml)	Accuracy RE (%)
Harpagide	600	575.51 ± 22.16	−4.08	654.47 ± 22.87	9.08	587.47 ± 36.62	−2.09	612.90 ± 22.28	2.15
	38.4	38.35 ± 3.75	−0.13	39.34 ± 1.92	2.45	38.05 ± 4.19	−0.92	40.68 ± 3.21	5.94
Chlorogenic acid	800	833.85 ± 63.43	4.23	816.58 ± 29.60	2.07	863.78 ± 21.49	7.97	847.20 ± 32.63	5.90
	51.2	53.43 ± 3.66	4.35	49.06 ± 4.43	−4.19	56.51 ± 2.77	10.38	51.69 ± 5.02	0.96
Swertiamarin	4800	4968.64 ± 208.19	3.51	5274.04 ± 147.92	9.88	5153.93 ± 132.15	7.37	4835.93 ± 153.29	0.75
	307.2	292.85 ± 19.45	−4.67	279.92 ± 15.61	−8.88	289.21 ± 13.27	−5.86	311.53 ± 14.38	1.41
Sweroside	1000	1035.71 ± 53.35	3.57	1113.06 ± 32.37	11.31	1079.40 ± 37.61	7.94	973.49 ± 29.27	−2.65
	64	60.97 ± 4.81	−4.74	66.44 ± 4.64	3.81	63.90 ± 2.67	−0.16	63.40 ± 2.49	−0.94
Angoroside C	200	185.46 ± 9.39	−7.27	203.84 ± 4.39	1.92	196.52 ± 13.15	−1.74	178.97 ± 6.04	−10.51
	12.8	13.02 ± 1.05	1.70	13.32 ± 1.09	4.09	13.53 ± 0.98	5.68	13.02 ± 1.17	1.73
Liquiritin	200	173.78 ± 5.96	−13.11	189.11 ± 4.81	−5.45	180.23 ± 4.39	−9.88	184.99 ± 6.52	−7.50
	12.8	13.43 ± 0.75	4.90	12.47 ± 0.97	−2.58	12.41 ± 0.76	−3.04	13.40 ± 0.70	4.70
Isoliquiritigenin	200	220.77 ± 9.12	10.39	192.67 ± 7.93	−3.66	223.78 ± 4.10	11.89	177.89 ± 4.50	−11.06
	12.8	13.27 ± 0.64	3.66	13.72 ± 0.54	7.15	13.83 ± 1.09	8.02	13.81 ± 0.76	7.87