Geiparvarin Inhibits OS Metastasis through Upregulation of ANGPTL4 Expression by Inhibiting miRNA-3912-3p Expression

Figure 4

ANGPTL4 is a target of miR-3912-3p in OS. (a) Predicted binding sites of miR-3912-3p in the wild-type 3-UTR of ANGPTL4. Mutations in this 3-UTR are highlighted in red. (b) Inhibitory effects of GN on miR-3912-3p expression were measured by qRT-PCR analysis. (c) Inhibitory effects of miR-3912-3p on ANGPTL4 mRNA expression was measured by qRT-PCR analysis. (d) Inhibitory effects of miR-3912-3p on ANGPTL4 protein level was measured by western blot. (e) Luciferase activity of the reporter driven by the wild-type or mutant 3-UTRs of ANGPTL4 in OS cells cotransfected with control oligonucleotides (NC) or miR-3912-3p mimics. (f) miR-1392-3p effects on GN induced upregulation of ANGPTL4 was measured by western blot.

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