Fu Fang Zhen Zhu Tiao Zhi Capsules Protect against Myocardial Ischemia by Inhibiting Cardiomyocyte Pyroptosis

Figure 2

FTZ inhibits pyroptosis in ischemic hearts. (a and b) Double-fl;uorescence staining with DAPI (blue) and TUNEL (green) and quantitative TUNEL data. Scale bar indicates 100 μm (right);n = 3. (c and d) Representative western blots (3 independent experiments) (left) and quantiflcation of western blots. β-actin was used as an internal control. (e) Quantitative RT-PCR for NLRP3, GSDMD, ASC, C-Caspase-1, 1L-1β, and 1L-18. (f and g) Serum IL-18 and IL-1β levels were measured using ELISA. , vs. sham; ^#, ^## vs. MI. (h) NLRP3 representative IHC images. Black arrows indicate the positions of NLRP3. Scale bar indicates 100 μm; n = 3.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)