Cloning and Prokaryotic Expression of Carotenoid Cleavage Dioxygenases from Mulberry (Morus notabilis)

Table 1

Primers used in this study.

Gene	Primer (5′-3′)	Used for
MnCCD1A	ATGGCGGAAGTGGCGTC	RT-PCR
	GATATTCAGTTTGAGTCCGAGTA
MnCCD1A	atggagctcggtaccATGGCGGAAGTGGCGTC	Prokaryotic expression vector construction
	caggtcgacaagcttgaattcGATATTCAGTTTGAGTCCGAGTA
MnCCD1B1	ATGGCCGAAATAGTGGATGTGAAT	RT-PCR
	TAGGACACCATTCCCAACATCAA
MnCCD1B1	atggagctcggtaccATGGCCGAAATAGTGGATGTGAAT	Prokaryotic expression vector construction
	caggtcgacaagcttgaattcTAGGACACCATTCCCAACATCAA
MnCCD1B2	GTGGGACTGTTCAAGGC	RT-PCR
	CTCTGCTGGGCAAATC
MnCCD1B2	atggagctcggtaccATGGCGGAGAAGCCGAGC	Prokaryotic expression vector construction
	caggtcgacaagcttgaattcGAGTTTTGCTTGTTCTTGCAGTTG
MnCCD4	ATGGCTTCCTCTTTTTTGGCAC	RT-PCR
	TCATGCCTGGTGAACCAAGTCC
MnCCD4	atggagctcggtaccATGGCTTCCTCTTTTTTGGCAC	Prokaryotic expression vector construction
	caggtcgacaagcttgaattcTCATGCCTGGTGAACCAAGTCC
Mn008739	ATGCCACATCACGTCTCCAA	RT-PCR
	CACCGAGACTGGTATGAAAGCT
Mn008739	atggagctcggtaccATGCCACATCACGTCTCCAA	Prokaryotic expression vector construction
	caggtcgacaagcttgaattcCACCGAGACTGGTATGAAAGCT
Mn008741	ATGGCATCATCGTATATGGCAT	RT-PCR
	TGGTGAGATTGGTATGAAAGCT
Mn008741	atggagctcggtaccATGGCATCATCGTATATGGCAT	Prokaryotic expression vector construction
	caggtcgacaagcttgaattcTGGTGAGATTGGTATGAAAGCT

Lowercase letters represent the homology arms containing Kpn I or ECOR I restriction sites.