The Experimental Exploration of TCM Theory “Treating the Same Disease with Different Approaches” on an Ulcerative Colitis Model

Table 3

PCR primer(s)

Gene	Sequence of primers (5′-3′)	Product size	Cycling parameters
TLR4	F: CTT TCA GGG AAT TAG GCT CC; R: CCA AGA TCA ACC GAT GGA C	116 bp	10 min at 95°C, 30sec at 95°C, 30sec at 55°C, 20 sec at 72°C, for 40 cycles
NF-κb	F: ATC TGT TTC CCC TCA TCT T; R: GTG CGT CTT AGT GGT ATC TG	167 bp	10 min at 95°C, 30sec at 95°C, 30sec at 55°C, 20 sec at 72°C, for 40 cycles
MIF	F: TCT CCG CCA CCA TGC CTA TG; R: GGG TCG CTC GTG CCA CTA AA	178 bp	10 min at 95°C, 30sec at 95°C, 30sec at 60°C, 20 sec at 72°C, for 40 cycles
MAdCAM-1	F: CCG AAA TCC ACC AGA ACC; R: TCC AAT GCA CCG TCA CTC	81 bp	10 min at 95°C, 30sec at 95°C, 30sec at 54°C, 20 sec at 72°C, for 40 cycles
GAPDH	F: CCA TGG AGA AGG CTG GG; R: CAA AGT TGT CAT GGA TGA CC	195 bp	10 min at 95°C, 30sec at 95°C, 30sec at 54°C, 20 sec at 72°C, for 40 cycles