Isoschaftoside Inhibits Lipopolysaccharide-Induced Inflammation in Microglia through Regulation of HIF-1α-Mediated Metabolic Reprogramming

Figure 1

Isoschaftoside downregulates lipopolysaccharide-stimulated expression of pro-inflammatory mediators. (a) CCK-8 assay shows that isoschaftoside did not have cytotoxic activities on BV2 cells within a range of concentrations. (b) The production of nitric oxide in the culture medium was quantified by Griess assay. (c–g) The cell lysates were obtained for expression of COX2, TNF-α, IL-1β and iNOS by western blot. The data are expressed as means ± SD; for comparisons shown, n = 6. Statistical analyses were done by two-way ANOVA with the Tukey’s post hoc test.

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