Isoschaftoside Inhibits Lipopolysaccharide-Induced Inflammation in Microglia through Regulation of HIF-1α-Mediated Metabolic Reprogramming

Figure 4

Isoschaftoside reduces HIF-1α and glycolytic enzymes production in CoCl₂-treated microglia. BV2 cells were pretreated with 100 μM CoCl₂ for 24 h followed by 200 μM isoschaftoside for 9 h. (a) Representative fluorescence images of HIF-1α was observed by immunofluorescence microscope. (Scale bar above: 250 μm, scale bar below: 25 μm). (b) Quantification of average fluorescence intensity of HIF-1α staining by ImageJ. (c–f) The expression of HIF-1α, HK2 and PFKFB3 by western blot. Data are expressed as means ± SD, ; ; for comparisons shown, n = 6. Statistical analyses were done by two-way ANOVA with the Tukey’s post hoc test.

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