Oat Seedlings Extract Inhibits RANKL-Induced c-Fos/NFATc1 Transcription Factors in the Early Stage of Osteoclast Differentiation

<div>Effect of the oat seedlings’ extract (OSE) on osteoclast differentiation. Bone marrow-derived macrophages (BMMs) were cultured in the presence of the receptor activator of nuclear factor <i>κ</i>B ligand (RANKL; 10 ng/mL) and the macrophage-colony stimulating factor (M-CSF; 30 ng/mL) for four days and treated with vehicle or the indicated concentration of OSE. (a) Visualization of multinucleated cells (MNCs) by using the tartrate-resistant acid phosphatase (TRAP) staining (10x magnification). (b) Counting of the TRAP-positive MNCs as osteoclasts. (c) Estimation of the TRAP activity at 405 nm. (d) Evaluation of cytotoxicity of the OSE by using the Cell Counting Kit-8. 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Evidence-Based Complementary and Alternative Medicine

fig1

Figure 1

Figure 1: Oat Seedlings Extract Inhibits RANKL-Induced c-Fos/NFATc1 Transcription Factors in the Early Stage of Osteoclast Differentiation 