Oat Seedlings Extract Inhibits RANKL-Induced c-Fos/NFATc1 Transcription Factors in the Early Stage of Osteoclast Differentiation

Figure 3

Effect of the oat seedlings’ extract (OSE) on signaling molecules involved in early osteoclast differentiation. Bone marrow-derived macrophages (BMMs) were pre-treated with the OSE (100 μg/mL) or vehicle for 1 h prior to the receptor activator of nuclear factor κB ligand (RANKL) stimulation (10 ng/mL) at the indicated time periods. Western blotting analysis of phosphorylated and total AKT, JNK, ERK, and p38 was performed. Actin was used as a loading control.