Oat Seedlings Extract Inhibits RANKL-Induced c-Fos/NFATc1 Transcription Factors in the Early Stage of Osteoclast Differentiation

Figure 4

Effect of the oat seedlings’ extract (OSE) treatment on osteoclastogenesis-mediated transcription factors. (a) The mRNA expression levels of the c-Fos, the nuclear factor-activated T cells c1 (NFATc1), and the c-Fos/NFATc1-dependent molecules (tartrate-resistant acid phosphatase (TRAP), osteoclast-associatedimmunoglobulin-like receptor (OSCAR), the dendritic cell-specific transmembrane protein (DC-STAMP), and the cathepsin K) as evaluated by real-time PCR. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as the internal control. Bone marrow-derived macrophages (BMMs) were stimulated with the receptor activator of nuclear factor κB ligand (RANKL; 10 ng/mL) and the macrophage-colony stimulating factor (M-CSF; 30 ng/mL) in the absence or presence of OSE (100 μg/mL) for the indicated times. ; ; (versus the vehicle control). (b) Analysis of expression of the RANKL-induced mediators by using western blot analysis after the OSE and vehicle treatment. Actin was used as the internal control. Data are representative of at least three independent experiments.

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