3β,23-Dihydroxy-12-ene-28-ursolic Acid Isolated from Cyclocarya paliurus Alleviates NLRP3 Inflammasome-Mediated Gout via PI3K-AKT-mTOR-Dependent Autophagy

Figure 5

Effects of compound 2 on intracellular ROS production and NLRP3 inflammasome activation. (a) The levels of intracellular ROS. (b) The protein expression levels of IL-1β and caspase-1 in the supernatants, as well as pro-IL-1β, pro-caspase-1, and NLRP3 in the cell lysates. (c) The relative expression levels of ROS. (d) The levels of IL-1β in the supernatants. (e) The relative expression levels of IL-1β, caspase-1, pro-IL-1β, pro-caspase-1, and NLRP3. The values are expressed as mean ± SD. ^## < 0.01 compared with the control group.  < 0.01 compared with the model group. ^& < 0.05 and ^&& < 0.01 compared with compound 2 (5 μM) group.

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