3β,23-Dihydroxy-12-ene-28-ursolic Acid Isolated from Cyclocarya paliurus Alleviates NLRP3 Inflammasome-Mediated Gout via PI3K-AKT-mTOR-Dependent Autophagy

Figure 7

Effects of the autophagy on intracellular ROS production and the expression and activation of the NLRP3 inflammasome. (a) The levels of ROS in the intracellular. (b) The relative expression level of ROS in MSU-induced THP-1. (c) The protein expression levels of IL-1β and caspase-1 in the supernatants, as well as pro-IL-1β, pro-caspase-1, and NLRP3 in the cell lysates. (d) The levels of IL-1β in the supernatants. (e) The relative expression levels of IL-1β, caspase-1, pro-IL-1β, pro-caspase-1, and NLRP3. The values are expressed as mean ± SD. ^## < 0.01 compared with the control group.  < 0.01 compared with the model group. ^& < 0.05 and ^&& < 0.01 compared with compound 2 (5 μM) group.

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