3β,23-Dihydroxy-12-ene-28-ursolic Acid Isolated from Cyclocarya paliurus Alleviates NLRP3 Inflammasome-Mediated Gout via PI3K-AKT-mTOR-Dependent Autophagy

Figure 8

Effects of the compound 2 on the PI3K-AKT-mTOR signal pathway. (a, c) The protein expression levels of PI3K, p-PI3K, AKT, p-AKT, mTOR, p-mTOR, LC3, and p62 by the Western blot. (b, d) The relative expression levels of p-PI3K/PI3K, p-AKT/AKT, p-mTOR/mTOR, LC3II/LC3I, and p62. (e) The protein expression levels of LC3 by immunofluorescence. (f) The relative expression levels of LC3. The values are expressed as mean ± SD. ^## < 0.01 compared with the control group.  < 0.05 and  < 0.01 compared with the model group. ^& < 0.05 and ^&& < 0.01 compared with compound 2 (5 μM) group.

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