Nigella sativa Oil Reduces LPS-Induced Microglial Inflammation: An Evaluation on M₁/M₂ Balance

Table 3

The raw data (mean ± SEM) for ELISA and gene expression and biochemical assays in the presence of LPS stimulation.

	Control	LPS	LPS + NSO (μg/ml)			NSO (μg/ml)
			10	20	40	40
PGE2
Mean	43.50	413.3	329.3	276.2	205.8	42.17
SEM	5.536	20.67	15.26	17.09	21.70	5.952
TNF-α
Mean	154.2	1113	847.5	621.2	353.2	145.0
SEM	11.73	64.11	33.85	30.51	32.57	13.14
IL-1β
Mean	117.2	885.0	658.2	395.0	219.0	118.2
SEM	5.759	29.90	19.17	11.50	14.67	8.332
IL-6
Mean	171.0	1050	773.8	588.2	413.3	174.8
SEM	6.826	34.13	13.88	20.77	6.427	7.414
TNF-α gene
Mean	1.000	7.420	5.650	4.141	2.354	0.9667
SEM	0.07821	0.4274	0.2257	0.2034	0.2171	0.08760
COX-2 gene
Mean	1.000	8.267	6.587	5.523	4.117	0.8433
SEM	0.1107	0.4133	0.3053	0.3419	0.4341	0.1190
IL-1β gene
Mean	1.000	8.850	6.580	3.948	2.190	1.182
SEM	0.05759	0.2990	0.1920	0.1152	0.1467	0.08332
IL-6 gene
Mean	1.000	6.560	4.837	3.675	2.583	1.093
SEM	0.04272	0.2135	0.08645	0.1298	0.04017	0.04633
iNOS gene
Mean	1.000	6.220	4.586	3.485	2.449	1.036
SEM	0.04050	0.2024	0.08196	0.1231	0.03809	0.04393
Arg-1 gene
Mean	1.006	0.6854	1.614	1.986	2.958	0.9970
SEM	0.04350	0.03525	0.06462	0.07948	0.1184	0.04415
iNOS/Arg-1
Mean	1.000	8.200	2.850	1.750	0.8333	1.050
SEM	0.05164	0.5727	0.1648	0.09574	0.04216	0.04282
NO
Mean	4.500	16.75	12.17	9.625	6.333	4.792
SEM	0.2661	0.8490	0.4014	0.2940	0.2108	0.6338
Urea
Mean	6.877	1.336	1.660	2.788	5.319	7.154
SEM	0.3452	0.2399	0.05658	0.08570	0.1708	0.5853
NO/urea
Mean	0.6648	14.83	7.382	3.465	1.185	0.6824
SEM	0.05456	2.726	0.3716	0.1325	0.06169	0.08993