Exosomal miR-221-3p Derived from Bone Marrow Mesenchymal Stem Cells Alleviates Asthma Progression by Targeting FGF2 and Inhibiting the ERK1/2 Signaling Pathway

Figure 5

The ERK1/2 signaling is involved in the regulation of the exosomal miR-221-3p/FGF2 axis on ASMC progression. (a), (b) TGF-β1-treated ASMCs were incubated with BMSCs-derived exosomes alone or together with transfection with pcDNA-FGF2. The protein levels of p-ERK1/2 and ERK1/2 were detected by western blot analysis. TGF-β1-treated ASMCs were incubated with BMSCs-derived exosomes in the absence or presence of ERK1/2 signaling activator TBHQ. (c), (d) The protein levels of p-ERK1/2 and ERK1/2 were detected by western blot analysis. (e) ASMC proliferation was measured by using the CCK-8 assay. (f), (g) ASMC migration was measured by transwell assay. (h)–(j) The protein expression of fibronectin and collagen III was analyzed by using Western blot analysis. Data from at least three independent experiments were presented as mean ± SD .

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