Caffeic Acid Phenethyl Ester Inhibits Basal Lipolysis by Activating PPAR-Gamma and Increasing Lipid Droplet-Associated Perilipin in Mature Rat Adipocytes

Figure 1

CAPE treatment inhibits glycerol release in a dose- and time-dependent manner in mature adipocytes. (a) Adipocytes were treated with DMSO (0.1%), db-cAMP (1 mM), or CAPE (1, 10, 30, 50, 100, 300, 500, and 1000 μM) for 2 h. The db-cAMP-treated group served as the positive control for glycerol release. (b) Adipocytes were treated with CAPE (100 μM) for 0, 30, 60, 90, 120, 150, and 180 min. (c) Cell viability was determined through an XTT assay. Adipocytes were incubated with 100 μM CAPE multiple times compared with the control (0-min) group and exhibited no cytotoxicity. Data are expressed as percentages of the control DMSO group or 0-min group and are presented as means ± standard errors (n = 3); and compared with the control DMSO group or 0-min group. NS: no statistical difference.

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