Research Article

Paclitaxel Resistance Modulated by the Interaction between TRPS1 and AF178030.2 in Triple-Negative Breast Cancer

Figure 4

TRPS1 is a binding target of AF178030.2. (a) RNA binding protein immunoprecipitation assay result showing the interaction between AF178030.2 and TRPS1. Upon immunoprecipitating TRPS1, the presence of AF178030.2 was tested by quantitative RT-PCR, and the enrichment versus the housekeeping mRNA GAPDH was calculated. Ab: antibody; IgG: immunoglobulin G. n = 4. (b) Comparison of TRPS1 protein level between MDA-MB-231R and MDA-MB-231P cells. (c) TRPS1 protein level after AF178030.2 overexpression. (d) TRPS1 protein level after knockdown of AF178030.2. versus control group. n = 5.