Phillyrin Prevents Ovariectomy-Induced Osteolysis by Inhibiting Osteoclast Differentiation

<div><i>Phillyrin</i> (<i>Phil</i>) attenuated osteoclast differentiation <i>in vitro</i>. (a) Representative tartrate-resistant acid phosphatase (TRAP) staining images from bone marrow macrophages (BMMs) treated with dimethyl sulfoxide (DMSO) or different doses of <i>Phil</i> in the presence of receptor activator for nuclear factor-<i>κ</i> B ligand (RANKL) and macrophage colony-stimulating factor (M-CSF) for 4 days. Scale bar = 200 <i>μ</i>m. (b, c) The area of TRAP-positive multinuclear osteoclasts were measured and the number was quantified (≥3 nuclei) in each well of the 96-well plate. 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Evidence-Based Complementary and Alternative Medicine

fig4

Figure 4

Figure 4: Phillyrin Prevents Ovariectomy-Induced Osteolysis by Inhibiting Osteoclast Differentiation 