Japanese Kampo Medicine Juzentaihoto Improves Antiviral Cellular Immunity in Tumour-Bearing Hosts

Figure 2

Depletion of Tregs, despite the lack of antitumour effects, may enhance anti-vaccinia cellular immunity in tumour-bearing mice. BALB/c mice were subcutaneously challenged with 1 × 10⁶ syngeneic CT26 cells. On day 14 after tumour exposure, mice were i.p. inoculated with 1 × 10⁷ PFU of recombinant vaccinia (vSC25). Simultaneously, some mice were also i.p. administered with 0.3 mg of anti-CD25 mAb. At 7 dpi with vSC25, the recovered splenocytes were subsequently stained with Abs against CD3, CD8β, and HIV-1 P18-I10 peptide-loaded H-2D^d tetramer. Flow cytometry was performed to determine the proportions of CD3⁺ CD8β⁺ H-2D^d/P18-I10⁺ cells. Tumour area is shown in (a). (b) shows representative flow data. The presented dot plots were obtained by gating the CD3⁺ population. (c) shows the cumulative data of two independent experiments (n = 4 for the CT26 group and n = 4 for the CT26 + anti-CD25 group). Data are expressed as mean ± standard deviation (SD). (Student’s t test) in the left panel of (c). (Student’s t test) in the right panel of (c).

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(b)

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