Japanese Kampo Medicine Juzentaihoto Improves Antiviral Cellular Immunity in Tumour-Bearing Hosts

Figure 4

JTT has no impact on the abundance of CD4⁺ CD25⁺ Foxp3⁺ Tregs and MDSCs in tumour-bearing mice after vSC25 inoculation. BALB/c mice were subcutaneously challenged with 1 × 10⁶ syngeneic CT26 cells and subsequently fed with either the control or JTT diet for 21 days. On day 14 after tumour injection, mice were i.p. inoculated with 1 × 10⁷ PFU of vSC25. At 7 dpi, the spleens were collected from the mice and recovered cells were subsequently stained with Abs against CD3, CD4, CD25, and Foxp3 (a and b) or against CD45, CD11b, Ly6G, and Ly6C (c and d). Flow cytometry was performed to measure the proportions of CD25⁺ Foxp3⁺ cells. The presented dot plots in (a) were obtained by gating the CD3⁺ CD4⁺ population. Flow cytometry was also performed to measure the abundance of Ly6G^- Ly6C^hi M-MDSCs and Ly6G⁺Ly6C^lo PMN-MDSCs. The presented dot plots in (c) were obtained by gating the CD45⁺ CD11b⁺population. (b) and (d) show cumulative data of two independent experiments. Each group consisted of 2-3 mice. Each symbol corresponds to one data point. The data are expressed as mean ± SD.

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