Deciphering the Metabolomics-Based Intervention of Yanghe Decoction on Hashimoto’s Thyroiditis

Figure 2

OPLS-DA model score scatter plot and differential metabolite analysis. (a) Scatter plot of OPLS-DA model score and volcano plot of differential metabolites for group M versus group T detected by mass spectrometry coupled with the high-performance liquid phase. (b) Scatter plot of OPLS-DA model score and volcano plot of differential metabolites for group M versus group T detected by mass spectrometry coupled with gas phase. The size of scattering in the volcano plot represents the VIP value of OPLS-DA model, the larger the scatter the larger the VIP value. Significantly upregulated metabolites are shown in red, significantly down-regulated metabolites are shown in blue, and nonsignificantly different metabolites are shown in gray. (c) Scatter plot of OPLS-DA model scores for group M versus group T detected by NMR. (d) OPLS-DA loadings plot for group M versus group (T). The horizontal coordinates in the plot indicate the magnitude of chemical shifts, from right to left, with increasing values of chemical shifts; the vertical coordinates indicate the magnitude of loadings after back conversion, where the shades of color indicate the magnitude of correlation coefficients, red indicates a positive correlation, and blue indicates negative correlation. The marked substances in the figure are the differential metabolites that passed the critical value test of the correlation coefficient.

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