[Retracted] FSCN1 Promotes Glycolysis and Epithelial-Mesenchymal Transition in Prostate Cancer through a YAP/TAZ Signaling Pathway

Figure 2

FSCN1 promotes prostate cancer cell invasion, migration, and EMT. (a) qRT-PCR for the detection of the mRNA expression of FSCN1 in PC-3 cells transfected with si-NC, si-FSCN1#1, and si-FSCN1#2, vs si-NC; (b, c) western blot for the examination of the protein expression level of FSCN1 in PC-3 cells after si-NC, si-FSCN1#1, and si-FSCN1#2 transfection, vs si-NC; (d) qRT-PCR to detect the mRNA expression level of FSCN1 in DU145 cells in groups of mock and FSCN1, ; (e, f) western blot for determining the protein expression level of FSCN1 in DU145 cells in the mock group and the FSCN1 group; (g) transwell to measure the invasion ability of PC-3 cells in groups of si-NC, si-FSCN1#, and si-FSCN1#2 and DU145 cells in groups of mock and FSCN1; (h) scratch test for evaluating the migration ability of PC-3 cells in the si-NC group, si-FSCN1#1 group, and si-FSCN1#2 group and DU145 cells in the mock group and the FSCN1 group; (i, j) western blot to determine the protein expression level of E-cadherin, vimentin, and snail in PC-3 cells in the si-NC group, si-FSCN1#1 group, and si-FSCN1#2 group, vs si-NC; (k, l) western blot for detecting the protein expression level of E-cadherin, vimentin, and snail in DU145 cells in the mock group and the FSCN1 group, .

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