Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma

Figure 3

MG induces apoptosis through activated caspase3 in mouse melanoma cells. (a) Annexin V assay showing apoptosis in B16F10 cells treated with MG for 12 h. (b) Western blot data showing protein expression of caspase3 and cleaved caspase3 in B16F10 cells 12 h after MG treatment. (c) Relative density of cleaved caspase3 normalized by β-actin. (d) Fluorescent images showing the activated caspase3 in B16F10 cells treated with MG after 24 h. Scale bar, 100 μm. (e) Quantification of the mean fluorescence intensity of cleaved caspase3. (f) Fluorescent images showing the activated caspase3 in PBS- or MG-treated mouse tumor tissue. Scale bar, 100 μm. (g) Quantification of the intensity of cleaved caspase3 in the mouse tumor region. MG, methyl gallate; Cl-cas3, cleaved caspase3. Values represent mean ± SD. ; ; versus untreated cells.

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