Methyl Gallate Suppresses Tumor Development by Increasing Activation of Caspase3 and Disrupting Tumor Angiogenesis in Melanoma

Figure 4

MG suppresses the cell migration and tube formation in B16F10 cells and inhibits the formation of blood vessels. (a) Images showing the cell migration on HUVECs after MG treatment. Magnification, 100×. Scale bar 50 μm. (b) Quantification of the number of migrated cells at 12 h. (c) Images showing the tube formation in HUVECs treated with MG after 12 h. Scale bar 200 μm. (d) Quantification of the number of tubules after MG treatment. (e) Fluorescent images showing the blood vessels density and pericyte coverage in PBS- or MG-treated mouse tumor tissue. Scale bar, 100 μm. Quantification of CD31 (f), and α-SMA (g) intensity in the mouse tumor region. MG, methyl gallate. Values represent mean ± SD. ; versus untreated cells or control.

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