[Retracted] CircAGFG1 Promotes Osteosarcoma Progression and Stemness by Competing with miR-302a-3p to Upregulate the Expression of LATS2

Figure 1

circAGFG1 drives osteosarcoma cell proliferation, migration, invasion, and metastasis. (a) The expression of circAGFG1 in osteosarcoma cell lines was significantly higher than that of FOB and NIH3T3 cells. (b) Detection of transfection efficiency by qRT-PCR. Compared with cells with empty vectors, the use of lentiviral shRNA can significantly reduce the level of circAGFG1. (c) Clone formation assays in U2OS and HOS cells showed that the number of clones was reduced in circAGFG1 knockdown cells compared to control cells. (d) The Matrigel-coated Transwell experiment showed that, when circAGFG1 was knocked down, the invasion potential of U2OS and HOS was significantly reduced. (e) CCK-8 experiment showed that, after knocking down circAGFG1, the proliferation ability of U2OS and HOS was significantly reduced. , . NC: negative control. n.s.: not significant.

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