[Retracted] CircAGFG1 Promotes Osteosarcoma Progression and Stemness by Competing with miR-302a-3p to Upregulate the Expression of LATS2

Figure 4

circAGFG1 as a posttranscriptional regulator. (a) RNA FISH shows that circAGFG1 is mainly located in the cytoplasm of osteosarcoma cells. (b) The predicted potential binding site between circAGFG1 and miR-302a-3p. (c) The dual-luciferase reporter gene verifies the binding of circAGFG1 and miR-302a-3p. Luciferase activity in 293T cells when circAGFG1 WT or Mut vector is cotransfected with miR-302a-3p mimic or negative control. The miR-302a-3p mimic reduced the luciferase activity of the circAGFG1-WT reporter vector but did not reduce the luciferase activity of the empty vector or the mutant reporter vector. (d) The predicted potential binding site between miR-302a-3p and LATS2. (e) The dual-luciferase reporter gene verifies the binding of LATS2 to miR-302a-3p. Luciferase activity in 293T cells when LATS2 WT or Mut vector is cotransfected with miR-302a-3p mimic. The miR-302a-3p mimic reduced the luciferase activity of the LATS2 WT reporter gene vector but did not reduce the luciferase activity of the LATS2 mutant reporter gene vector. (f) Detection of miR-302a-3p expression in tumor tissues by qRT-PCR. Compared with cells with empty vectors, the use of lentivirus shRNA can significantly increase the level of miR-302a-3p. . n.s.: not significant.

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